3DNA Blot Probe Kits offer superior performance and reliability for Northern and Southern analyses. Our EasyAP kit offers both the sensitivity and reliability of ³²P as well as a protocol that is as easy as a ³²P protocol and produces results much more quickly.

Detection of the Follicle Stimulating Hormone Receptor gene

Human genomic DNA was restricted with EcoR1, loaded onto gels at the concentrations indicated, electrophoresed, and blotted onto membranes. The left membrane was probed with a 3DNA EasyAP probe and detected with chemiluminescence (20 minute film exposure). The right membrane was probed with a ³²P labeled random primed cDNA (17 hour film exposure).

	Sensitive: Detect single copy genes in 0.2µg of human genomic DNA; detect less than 1.5fg of target
	Reliable: 3DNA probe technology provides consistent results
	Simple: Straightforward procedure compatible with all standard hybridization protocols
	Convenient: Direct alkaline phosphatase or digoxigenin detection chemistries; 32P kits also available
	Specific: Probe short, precise nucleotide sequences

3DNA™ - the ideal non-isotopic detection system
3DNA non-isotopic probes represent a new generation of hybridization tools with enhanced sensitivity and reliability. Now you can use non-isotopic labeling and routinely detect a single copy gene in < 0.2µg of human genomic DNA in a Southern blot or detect low abundance mRNAs in a Northern blot. Further, you can detect and distinguish between low abundance mRNA sequences that differ by one base because a 3DNA probe's target sequence specificity is determined by an oligonucleotide. With 3DNA products, you get results you cannot get by any other method in Southern, Northern, or library screening experiments.

Signal enhanced by 3DNA dendrimer technology
The basis for this unprecedented performance is 3DNA dendrimer technology. The highly branched 3DNA dendrimer is a scaffold decorated with hundreds of biotin or digoxigenin molecules along with multiple target-specific oligonucleotide probes. You can create 3DNA probes in your own lab from your own oligonucleotides (specific 3' sequence required) or have Genisphere make one to your specifications.

Mutation analysis - detect single base mismatches
3DNA probes as short as 18 bases can be used to detect single base changes in mutations and polymorphisms. They can be directed to extremely well-defined regions of a gene or RNA and used for fine-structure gene mapping, to follow the expression of closely related allelic genes, to check the integrity of transfected-expressing genes, or to screen genomic or cDNA libraries. They are ideal for high-throughput screening including high density colony arrays.

Standard Kit or Starter Kit Formats
3DNA Probe Kits come in Standard or Starter sizes. The Standard kits have sufficient reagents to ligate up to 4 different oligonucleotides and to hybridize up to 2000 cm² of membrane. The Starter kits have sufficient reagents to ligate up to 2 different oligonucleotides and hybridize 1000 cm² of membrane. All 3DNA kits follow a simple, straightforward procedure that is compatible with all standard hybridization protocols.

Expression Array Detection | FISH Probes | Blot Probes | 3DNA Products