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RNA Amplification RNA Amplification SenseAMP Plus Low Molecular Weight Kit RampUP/RampUP Plus Kit Ordering Information FAQs SenseAMP/SenseAMP Plus Kit Protocols Data and References
RNA Amplification
SenseAMP Kit

Catalog Numbers
SenseAMP* 10 assays SENSEAMP10
SenseAMP* 20 Assay SENSEAMP20
cDNA Synthesis 10 Assay CDNAMOD
SenseAMP Plus** 10 Assay SENSEAMP10P
SenseAMP Plus** 20 Assay SENSEAMP20P
Plus** 10 Assays P10PLUS
 

*cDNA Synthesis Kit is required for gene expression analysis of senseRNA from RampUP and SenseAMP on Affymetrix GeneChips or using direct incorporation of a fluorescent - or aminoallyl-dUTP. SenseRNA is reverse transcribed incorporating labeled-dUTP to generate labeled cDNA for hybridization (labeled nucleotide not included).

**If using Array 50, Array 350 or Array 900 to label poly(A)-tailed senseRNA (senseRNA modified with the Plus module), you will need to purchase the following high-concentration primers: Primer-30 pmol-Cy3, Alexa 546, Oyster 550 (cat. no. CMR960UA) Primer-30 pmol-Cy5, Alexa 647, Oyster 650 (cat. no. CMR960UB)

Order SenseAMP Kit Now

The most accurate amplification method for intact or degraded samples.

 
RNA Amplification - SenseAMP
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Features and benefits:
 
  Higher Fidelity Amplification
    When compared to unamplified RNA by qRT-PCR analysis, SenseAMP sRNA has a higher correlation (r2 = 0.93) than aRNA produced by conventional Eberwine amplification (r2 = 0.86).
   
  Amplification of Degraded and Prokaryotic RNA
    SenseAMP has the unique ability to amplify degraded and prokaryotic RNA , including RNA extracted from paraffin-embedded samples.
     
  Full-Length Amplification
    SenseAMP produces nearly full-length transcripts, avoiding the 3 ’ bias of other amplification methods.
     
  Sense-Strand Orientation
    SenseAMP produces RNA in the original “sense” orientation which can be labeled using the conventional methods used with unamplified samples.
     
  Faster, Easier Protocol
    Because SenseAMP kits do not employ second-strand synthesis, they have a faster and easier protocol than conventional amplification kits.
     
View Application Note: Use of Formalin Fixed, Paraffin-Embedded RNA Samples in Amplification and Labeling Methods for Gene Expression Analysis.
 
PLUS more….
 
SenseAMP Plus has the same features and benefits as SenseAMP, and includes the PLUS Module for poly(A) tailing, making the senseRNA compatible with any protocol that uses dT priming.
 
Features and benefits:
 
  Compatibility with dT Priming Protocols
    SenseAMP Plus produces polyadenylated RNA in the original “sense” orientation as dT-primed labeling protocols.
     
  Amplification of Any RNA
    SenseAMP Plus has the unique ability to amplify LMW RNA samples including microRNA, piRNA, snRNA, hnRNA, etc.
     
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