The most accurate linear amplification kit for low molecular weight RNA samples including severely degraded RNA, piRNA, miRNA, snRNA, hnRNA, etc.
SenseAMP Plus Amplification of LMW RNA
Fidelity of Amplification
Experimental Design:
Rat Brain and Liver Total RNA were enriched for LMW RNAs with YM100 columns. The LMW RNA enriched from 1ug total was amplified with the SenseAMP Plus LMW kit (Genisphere). After amplification, 17ug senseRNA was obtained for the Brain sample, and 13ug senseRNA was obtained for the Liver sample. 250ng of these amplified RNAs were labeled with FlashTag, and hybridized to NCode microarrays. Parallel FlashTag labeling reactions and hybridizations were run with the LMW RNA from 0.75ug Total RNA (no RNA amplification). The Brain/Liver differentials were compared.
Features and benefits:
High Fidelity Amplification
The expression patterns of unamplified and amplified LMW RNAs show high correlation (r2=0.93).
Excellent Reproduciblity
Parallel amplification reactions of the same LMW RNA sample have a high correlation coefficient (r2= 0.9884).
Low requirements of input LMW RNA
Only 0.5-30ng of input LMW RNA is required for SenseAMP Plus amplification.
