The capture antibody is diluted in buffer and incubated overnight in the wells. Unbound antibodies are washed away and wells are blocked. Standards and samples are applied to the wells, incubated, and excess washed away. The biotinylated detection antibody is then incubated with samples and standards and excess washed away. Finally, UltraAmp Anti-Biotin HRP (200) is applied to the wells, incubated, and excess washed away. TMB HRP substrate is added to produce the signal. Signal is detected on an ELISA plate reader. With so many HRP molecules on the UltraAmp reagent, the sensitivity may be amplified up to 200-fold over standard Streptavidin-HRP detection.

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Fluorescent beads containing capture antibodies are added to each well, followed by the addition of celllysates. The beads and cell lysates are incubated overnight. The wells are washed and biotinylated detection antibodies are added, incubated, and excess washed away. Streptavidin-Phycoerthyrin (PE) is added, incubated, and excess washed away. Next, UltraAmp Anti-PE Biotin (900) is added to the wells, incubated, and excess is washed away. Streptavidin-PE is added for detection. Samples are analyzed using a fluorescence based flow detection instrument. Sensitivity may be increased up to 500-fold over standard Streptavidin-PE detection.

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Capture antibodies are spotted onto a coated glass slide. The array is blocked and washed. Sample and control antigens are prelabeled with FITC and Biotin, then hybridized to the array. After washing, UltraAmp Anti-FITC Oyster-650®(900) and UltraAmp Anti-BiotinOyster-550®(900) are hybridized. The slide is washed and scanned. UltraAmp detection reagents may increase sensitivity over standard fluorescently labeled detection antibodies up to 100-fold.

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Nucleic acid sequences are spotted onto a coated glass slide. Nucleic acid samples are prepared having UltraAmp capture tags, complementary to those attached to the UltraAmp reagent. The tags can be incorporated by a variety of methods including ligation, priming, and PCR. The tagged nucleic acid samples are hybridized to the array overnight. After washing, UltraAmp Capture Sequence 03 Oyster-550 (900) and UltraAmp Capture Sequence 35 Oyster-650 (900) are hybridized. Excess material is washed away and the array is scanned. With 900 fluorescent dyes on each dendrimer, the UltraAmp reagents may increase sensitivity over standard fluorescent labeling methods up to 50-fold.

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