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How many times can I reuse the glass slides? |
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Indefinitely, as long as the slides are washed thoroughly but not broken. |
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Do I need to do anything to dispose of the used spots? |
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These spots can be wrapped by a paper towel and then disposed of in your normal trash. |
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Can I buy a refill without the glass slides? |
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Yes, a refill kit for 75 reactions is available. It contains all 6 genes and two bottles of hybridization solutions.
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Are there multiple scenarios or just one? |
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At this point, we only have one but others are under development. Check the Gene Chip Genes to Disease and GCAT web pages for updates. |
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Can I buy a kit with different spot patterns or color combinations? |
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These are under development as well. |
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Why are the spots pink and blue instead of green and red? |
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The simulation uses dyes instead of fluorescent tags used in actual microarray experiments. Using these dyes saves on cost and improves the probability of student success in lab. |
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Why do the colors change with time? |
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The red dye is light sensitive and will fade to clear. We have modified the solutions to minimize this, but we cannot change the physical properties of the pH indicators. |
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Can I convert these colors to ratios? |
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Yes, as with real DNA microarray, the Gene Chips: Genes to Diseases simulation allows you to convert qualitative data to quantitative data. You can use the color scale to determine the ratio of your colors. For more information on quantification, go to this URL <www.bio.davidson.edu/people/macampbell/LRSD/colors.html> which was developed by Malcolm Campbell and Laurie Heyer. Over time, more curricular materials will be added to this page, and the GCAT web site. |
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Are there multiple scenarios or just one? |
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At this point, we only have one but others are under development. Check the Gene Chip Genes to Disease and GCAT web pages for updates. |
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