| All Genisphere reagents are carefully checked for quality before shipment to customers. Genisphere labeling kits are designed to achieve excellent microarray results. The sensitivity and performance of the kits is influenced by factors such as the quality of both the array and sample and technique. Good microarray results can be achieved by considering the following tips: |
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Properly store and handle kit components and other reagents. DO NOT use expired reagents. |
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Follow the recommended procedures outlined in the protocols. Any modifications may affect results. |
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Check the purity and quantity of RNA prior to each use. Contaminants and miscalculations of RNA input can adversely affect the results. Always handle RNA with extreme care, avoiding any exposure to ribonucleases. |
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Check the purity and quantity of synthesized cDNA using gel electrophoresis or other quantitative methods. |
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If using custom arrays, confirm viability prior to using Genisphere labeling kits. |
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Scan arrays prior to hybridization to determine inherent background characteristics. |
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If using Agilent arrays, please call Technical Support for recommendations. |
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| In the event that a problem should arise, this guide is designed to help identify and resolve it quickly. Each section pertains to a specific problem area: procedural, background, signal and data. Under these categories, from left to right, are listed symptoms, causes and resolutions to many of the most common microarray problems. If a particular problem is not listed in this guide, please contact Genisphere Technical Support at (215) 996-3040 (M-F, 8:30am to 5pm EST) for further assistance. |
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