| |
Array
50 |
Array
350 |
Array
900 |
Array
900MPX |
| Using Total RNA |
| Quality of results |
Excellent |
Excellent |
Excellent |
Excellent |
| Quantity of mammalian RNA required |
10-20 µg |
1-5 µg |
0.25-1 µg |
0.5-2 µg |
Quantity of plant RNA
required |
20-40 µg |
2-10 µg |
0.5-2 µg |
1-4 µg |
| Quantity of bacterial RNA required |
N/A |
N/A |
N/A |
0.5-2 µg |
| |
| Using Poly(A)+ RNA |
| Quality of results |
Very good |
Very good |
Very good |
Excellent |
| Quantity of mammalian RNA required |
0.5-1 µg |
50-250 ng |
12.5-50 ng |
25-100 ng |
Quantity of plant RNA
required |
0.5-1 µg |
50-250 ng |
12.5-50 ng |
25-100 ng |
| |
| Using aRNA |
| Quality of results |
N/A |
N/A |
N/A |
Excellent |
| Quantity of mammalian RNA required |
N/A |
N/A |
N/A |
25-100 ng |
Quantity of plant RNA
required |
N/A |
N/A |
N/A |
25-100 ng |
| Quantity of bacterial RNA required |
N/A |
N/A |
N/A |
25-100 ng |
| |
| Use with degraded samples |
No |
No |
No |
Yes |
| |
| Use on cDNA arrays |
Yes |
Yes |
Yes |
Yes |
| |
| Use on oligo arrays |
Yes |
Yes |
Yes |
Yes |
| |
| Method of priming |
dT
Primer |
dT
Primer |
dT
Primer |
Random & dT Primer |
| |
| “Hands-on” time for labeling |
30 Min |
30 min |
15 min |
75 min |
| |
| Total time required for labeling |
3 hours |
3 hours |
2.5 hours |
5 hours |
| |
| Contains LNA dT blocker |
Yes |
Yes |
Yes |
Yes |
| |
| Labeling of genomic contamination |
None |
None |
None |
Minimal |
