Catalog Numbers
Array 900miRNA RT (for arrays printed with sense oligos)
MIRT130 Array 900miRNA RT Cy3 (200 Assays)
MIRT140 Array 900miRNA RT Cy5 (200 Assays)
The first high-sensitivity microarray labeling kit designed specifically for use with microRNA, siRNA, and other small RNA molecules (tRNA, 5S rRNA, nuclease fragments, etc). Using Genisphere's proprietary 3DNA signal amplification technology, Array 900miRNA RT kit produces accurate, validated results from only 50-200ng of microRNA enriched samples. The Array 900miRNA RT kit is for use on microarrays containing sense probes only.
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Exceptional Sensitivity |
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High-quality expression data can be generated using microRNA enriched from just 0.5-5ug of total RNA, or using microRNA enriched from 100 cells that is first amplified with Genisphere’s SenseAMP Plus RNA Amplification Kit. |
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Accurate Expression Measurement |
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Kit accuracy has been validated by Northern blots.
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Universal RNA Labeling |
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While the Array 900miRNA RT labeling kits are optimized to label microRNA, they have universal application to label any RNA molecule with a free 3' hydroxyl group. The kits can be used to label total RNA, poly (A) RNA, non-coding RNA, fragmented or degraded RNA, and low molecular weight RNAs including microRNA, siRNA, tRNA, and 5s rRNA. |
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Ease of Use |
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Easy, quick protocols with prelabeled detection reagents included. |
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View AACR poster: A Sensitive Method for MicroRNA Gene Expression Profiling Applied to Prostate Cancer Tissue and Cell Lines LNCaP and PC3 |
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Array 900miRNA RT Labeling Kit Data Set |
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| Comparison of microRNA |
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Comparison of microRNA expression of
microRNA extracted from frozen (Cy3) and
FFPE (Formalin Fixed, Paraffin Embedded)
(Cy5) Diffuse Large B-cell Lymphoma Case,
100 ng of enriched microRNA per channel
labeled with Array 900miRNA RT kits and
hybridized to Sense Strand microRNA chips. |
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