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Comparison of the Genisphere Kits' LNA dT blocker to standard oligo dT blocker
 
A series of three experiments was run on mouse cDNA arrays to compare the effects of different dT blockers on the observed differential. Experiments were run with no dT blocker, standard dT 70 blocker, and Genisphere's new LNA dT blocker. The experiments show that use of the LNA blocker increases the number of spots producing significant differential by 75% compared to standard dT blocker
 
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Comparison of Brain (Cy™3) vs. Liver (Cy™5)
Condition Hyb. Buffer Genes >50 fold Genes >25 fold Genes >10 fold Genes >5 fold Genes >2 fold
No dT Blocker Vial 6 @64C 5 21 65 129 363
Std dT70 Blocker Vial 6 @64C 5 31 95 202 463
LNA dT Blocker
(Vial 6 buffer)		 Vial 6 @64C 8 61 173 348 783
LNA dT Blocker (Vial 7 buffer) Vial 7 (30% Form.)@ 55C 21 60 175 357 760
 
Data based on average of duplicate spots
~4500 Genes spotted in duplicate
~2100 Genes giving significant signals
 
Effect of Choice of dt Blocker on Differential
 
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Go to Page 4 - Comparison of Brain (Cy3) vs. Liver (Cy5) Go to Page 3 - Genisphere Submicro Mouse cDNA Arrays - Augmented dT (LNA) Blocker Go to Page 2 - Genisphere Submicro Mouse cDNA Arrays - Standard dT70 Blocker Go to page 1- Genisphere Submicro Mouse cDNA Arrays - No dT Blocker
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