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FFPE sample used successfully with Affymetrix


		FFPE derived RNA successfully profiled on Affymetrix® 3' Expression Arrays using Genisphere Amplification and Labeling

A new dataset for the microarray community!

We used intact and FFPE RNA samples in our RampUP and SenseAMP kits for RNA amplification, and hybridized to Affymetrix® 3’ expression arrays and exon arrays. Download and view the dataset for more details!

		RampUP can be used to amplify and label both FFPE and Frozen RNAs. For Frozen RNAs, RampUP data is similar to Affymetrix data.

		RampUP enables the ability to resolve similar data from FFPE samples compared with similar fresh frozen samples.

		When comparing FFPE RNAs to matched fresh RNAs, Genisphere has excellent overlap of fresh/FFPE differentials.

Affymetrix is a registered trademark of Affymetrix, Inc.

Marligen Biosciences now distributes Genisphere’s labeling kits for detection of microRNAs on the Luminex® xMAP multiplex platform. The Vantage™ product line includes kits for purifying, labeling, and detecting microRNA. Marligen offers a complete system enabling throughput capabilities of over 100 samples a day, and is compatible with total RNA or enriched RNA including degraded RNA from archived tissues.

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Genisphere has taken small 3DNA dendrimers and labeled them with fluorescent dyes, Oyster®-550 and Oyster®-650. These fluorescently labeled 3DNA dendrimers are conjugated to Streptavidin, allowing for detection of biotinylated targets. With 40 fluorescent dyes per Streptavidin, these UltraAmp products offer significant signal amplification over common Streptavidin-dye conjugates.

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Endless possibilities for more sensitive detection in both Protein and Nucleic Acid assays! If you need more signal in protein arrays, bead-flow assays, or ELISAs, our Signal Amplifiers may be the solution. A range of targeting moieties and labels are available. The sensitivity improvement obtained with this single tube reagent is up to 200 fold, depending on the size of dendrimer, application, and nature of the assay.

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